MTL Annual Research Report 2013

Polina Anikeeva

Title: Professor
Department: Department of Materials Science and Engineering

Collaborators

Y. Fink, RLE, DMSE, MIT
E. Bizzi, McGovern Institute for Brain Research, MIT

Graduate Students

A. Canales, RA, DMSE
R. Chen, RA, DMSE
M.G. Christiansen, RA, DMSE
C. Lu, RA, DMSE

Support Staff

A. Inserto, Administrative Assistant II

Publications

Liske, H., Towne, C., Anikeeva, P., Zhao, S., Feng, G.,Deisseroth, K., Delp, S., Optical inhibition of motor neuron and muscle activity in vivo. Muscle and Nerve, 2012.

Anikeeva, P., Deisseroth, K., Photothermal genetic engineering. ACS Nano 26, 7548- 7552, 2012.

Anikeeva, P., Andalman, A.S., Witten, I.B., Warden, M.R., Goshen, I., Grosenick, L., Gunaydin, L.A., Frank, L., Deisseroth, K., Optetrode: a multichannel readout for optogenetic control in freely moving mice, Nat. Neurosci. 15, 163, 2011.

Witten, I.B., Lin, S.C., Brodsky, M., Prakash, R., Diester, I., Anikeeva, P., Gradinaru, V., Ramakrishnan, C., Deisseroth, K. Cholinergic interneurons control local circuit activity and cocaine conditioning. Science, 330, 1677, 2010.

Shirasaki, Y., Anikeeva, P.O., Tischler, J.R., Bradley, M.S., Bulović, V.Efﬁcient Förster energy transfer from phosphorescent organic molecules to J-aggregate thin ﬁlms, Chem. Phys. Lett., 485, 243, 2010.

Panzer, M.J., Aidala, K.E., Anikeeva, P.O., Halpert, J.E., Bawendi, M.G., Bulović V. Nanoscale morphology revealed at the interface between colloidal quantum dots and organic semiconductor films. Nano Lett. 10, 2421, 2010.

Anikeeva, P.O., Halpert J. E., Bawendi, M.G., Bulović, V. “Optimized Materials for High Performance QD-LEDs with Electroluminescence Tunable over Entire Visible Spectrum,” Nano Lett., 9, 2009.

Hummon, M.R., Stollenwerk, A.J. , Narayanamurti, V. , Anikeeva, P.O., Panzer, M.J., Wood, V.C., Bulović, V. Detecting Charging Energy and Charge State of CdSe/ZnS Quantum Dots using a Scanning Tunneling Microscope. Phys. Rev. B, 2009.

Anikeeva, P.O., Madigan, C.F., Halpert, J.E., Bawendi, M.G., Bulovic, V. Electronic and Excitonic Processes in Hybrid Organic-Quantum Dot LEDs, Phys. Rev. B 78, 2008.

Kim, L., Anikeeva, P. O., Coe-Sullivan, S. A., Steckel J. S., Bawendi, M. G., Bulović, V. Contact Printing of Quantum Dot Light Emitting Devices, Nano Lett., 8, 2008.

Anikeeva, P.O., Halpert J. E., Bawendi, M.G., Bulovic, V. Electroluminescence from a Mixed Red-Green-Blue Colloidal Quantum Dot Monolayer, Nano Lett. 7, 2196, 2007.

Anikeeva, P.O., Madigan C. F., Coe-Sullivan, S. A., Steckel, J.S., Bawendi, M.G., Bulovic, V. Photoluminescence of CdSe/ZnS core/shell quantum dots enhanced by energy transfer from a phosphorescent donor, Chem. Phys. Lett. 424, 120, 2006.

Steckel, J.S., Snee, P., Coe-Sullivan, S. A., Zimmer, J.P., Halpert, J. E., Anikeeva, P.O., Kim, L., Bulovic, V., Bawendi, M.G. Color-Saturated Green-Emitting QD-LEDs, Angew. Chem. Int. Ed. 45, 5796, 2006.

Ivanov, S.A., Nanda, J., Piryatinski, A., Achermann, M., Balet, L.P., Bezel, I. V., Anikeeva, P.O., Tretiak, S., Klimov, V. I. Light Amplification Using Inverted Core/Shell Nanocrystals: Towards Lasing in Single-Exciton Regime, J. Phys. Chem. 108, 10625, 2004.

Flexible Probes for in vivo Recording and Stimulation of Neural Tissue

Authors: A. Canales, U. P. Froriep, X. Jia, C. Lu, Y. Fink, P. Anikeeva
Sponsorship: NSF through MRSEC Grant DMR-0819762, NSF CSNE, NSF CAREER, McGovern Institute for Brain Research

Probing and stimulating neural tissue is important to understand fundamentals of information processing and pathologies of the nervous system such as Parkinson’s disease, epilepsy, or spinal cord injury. Therefore it is desirable to collect data from multiple recording sites with high resolution and specifically manipulate neuronal activity in the regions of interest. Moreover, unspecific interaction with the probed tissue should be reduced to a minimum.

Current technologies to achieve this collectionrecord and stimulate the nervous system are usually based on materials such as silicon, silica, or metals, which suffer from fundamental physical limitations: low signal-to-noise ratio, low resolution, and deterioration of the quality of recordings^[1].

We present a novel method to fabricate devices for high-resolution recording, optogenetic stimulation, and pharmacological interrogation of neural tissue. In contrast to lithographically fabricated devices based on silicon or metal wires, our method results in highly flexible and more biocompatible devices, addressing the need for long-term recordings with limited interaction of implanted probes with the tissue. Employing a thermal drawing process inspired by optical fiber production, we create a scalable tool combining recording and intervention in neurophysiology. Our initial experiments demonstrate single-neuron recording and optogenetic stimulation of activity in the medial prefrontal cortex in vivo (Figure 1).

Figure 1: Neural recordings obtained from the medial prefrontal. (A) Activity in absence of stimulation. (B) Spike shapes isolated from data in (A). (C) Response to 20-Hz optical stimulation. (D) The multi-unit spikes in response to optical pulses across 20 trials identical to the one shown in (C). (E) Firing rate averaged across 20 trials shown in (D). (F) Response to 100-Hz optical stimulation. (G) The multi-unit spikes in response to optical pulses across 20 trials identical to one shown in (F). (H) Firing rate averaged across 20 trials shown in (G).

M. P. Ward, P. Rajdev, and C. Ellison, “Toward a Comparison of Microelectrodes for Acute and Chronic Recordings,” Brain Research, Vol. 1282, pp. 183-200, 2009. [↩]