MTL Annual Research Report 2011

Collaborators

• R. Sobol, University of Pittsburgh Cancer Institute
• S. Bhatia, Harvard-MIT Division of Health Science and Technology
• P. Hammond, ChemE

Senior Postdoctoral Associate

• O. Kiraly

Graduate Students

• J. Ge, Research Assistant, BE
• M. Parrish, Research Assistant, BE
• M. Sukup Jackson, Research Assistant, BE
• D. Weingeist, Research Assistant, BE

Support Staff

• K. Reposa, Administrative Assistant II
• P. Mazzucato, Lab Technician

Publications

Engelward, B.P., Dreslin, A., Christensen, J., Huszar, D., Kurahara, C., and Samson, L.,  Repair deficient 3-methyladenine DNA glycosylase homozygous mutant mouse cells have increased sensitivity to alkylation induced chromosome damage and cell killing, EMBO J., 15: 945-952 (1996).  PMCID: PMC450292

Engelward, B.P., Weeda, G., Wyatt, M.D., Broekhof, J.L.M., de Wit, J., Donker, I., Allan, J.M., Gold, B.,Hoeijmakers, J.H.J., and Samson, L.D., Base excision repair deficient mice lacking the Aag alkyladenine DNA glycosylase, Proc. Natl. Acad. Sci. USA, 94: 13087-13092 (1997). PMCID: PMC24267

Engelward, B.P., Allan, J.M., Dreslin, A.J., Kelly, J.D., Wu, M.M., Gold, B., and Samson, L.D., A chemical and genetic approach together define the biological consequences of 3-methyladenine lesions in the mammalian genome, J. Biol. Chem., 273: 5412-5418 (1998).  PMID: 9479003

Hendricks, C.A., Razlog, M., Matsuguchi, T., Goyal, A., Brock, A.L., and Engelward, B.P., The S. cerevisiae Mag1 3-methyladenine DNA glycosylase modulates susceptibility to homologous recombination, DNA Repair, 1: 645-659 (2002).  PMID: 12509287

Hendricks, C.A.,  Almeida, K., Stitt, M.,  Jonnalagadda, V.,  Kerrison, F., Rugo, R.,Engelward,B.P.,Spontaneous mitotic homologous recombination at an EYFP direct repeat in transgenic mice, Proc. Natl. Acad. Sci. USA, 100: 6325-6330(2003).  PMCID: PMC164445

Kiziltepe, T., Yan, A., Dong, M., Jonnalagadda, V.S., Dedon, P.C., and Engelward, B.P., Delineation of the chemical pathways underlying nitric oxide-induced homologous recombination in mammalian cells, Chem. Biol., 3: 357-369 (2005).  PMID: 15797220

Jonnalagadda, V.S., Matsuguchi, T., and Engelward, B.P., Interstrand crosslink-induced homologous recombination carries an increased risk of deletions and insertions, DNA Repair, 4: 594-605 (2005).  PMID: 15811631

Rugo, R.E., Almeida, K.H., Hendricks, C.A., Jonnalagadda, V.S., and Engelward, B.P., A single acute exposure to a chemotherapeutic agent induces hyper-recombination in distantly descendant cells and in their neighbors, Oncogene, 25:5016-5025 (2005). PMID: 15856014

Wiktor-Brown, D.M., Hendricks, C.A., Olipitz, W., and Engelward, B.P., Age dependent accumulation of recombinant cells in the mouse pancreas revealed by in situ fluorescence imaging, Proc. Natl. Acad. Sci. U.S.A., 103:11862-11867 (2006).  PMCID: PMC1567667

Wiktor-Brown, D.M., Olipitz W., Hendricks C.A., Rugo R.E., and Engelward B.P., Tissue specific differences in the accumulation of sequence rearrangements with age, DNA Repair, 7:694-703 (2008).  PMCID: PMC3014828

Wiktor-Brown, D.M., Kwon H.S., Nam Y.S., So P.T., and Engelward B.P., Integrated one- and two-photon imaging platform reveals clonal expansion as a major driver of mutation load, Proc. Natl. Acad. Sci. U. S. A., 105:10314-10319 (2008).  PMCID: PMC2492490

Niu, Y., Wang H., Wiktor-Brown D.M., Rugo R., Shen H., Huq S., Engelward B.P., Epperly M., and Greenberger J.S., Irradiated esophageal cells are protected from radiation-induced recombination by MnSOD gene therapy, Rad. Res., 173:453-61 (2010).  PMCID:PMC2872095

Olipitz, W., Hembrador S., Davidson M., Yanch J.C. and Engelward B.P., Development and characterization of a novel variable low-dose rate irradiator for in vivo mouse studies, Health Phys. J., 98:727-34 (2010).  PMCID:PMC3020895

Wood, D.K., Weingeist D.M., Wu Y., Bhatia S.N., and Engelward B.P. Single cell trapping and DNA damage analysis using microwell arrays, Proc. Natl. Acad. Sci. U.S.A.,107:10008-13 (2010).  PMCID:PMC2890454

Rugo, R.E., Mutamba J.T., Mohan K.N., Yee T., Chaillet J.R., Greenberger J.S., and Engelward B.P.  Methyltransferases mediate cell memory of a genotoxic insult, Oncogene, 30: 751–756 (2011).  PMCID: PMC3044496