MIT Summer Course in Molecular and Cell Biology for Engineering Faculty

June 17 - 21, 1996

This course was organized by Prof. Douglas A. Lauffenburger on behalf of faculty in the MIT School of Engineering who wished to learn about modern molecular and cell biology.

Participants

Photograph taken June 21, 1996. With a suitable browser you can read the names of the people in this photograph with your cursor.

Left to right, standing: T. Alan Hatton (ChE), L. Mahavadan (ME), Gregory Rutledge (ChE), Paul E. Laibinis (ChE), Paul I. Barton (ChE), Clark K. Colton (ChE), John B. Vander Sande (MSE), Linn W. Hobbs (MSE), Preetinder S. Virk (ChE), Monty Krieger (Biology), Wolf W. von Maltzahn (Whitaker Foundation), Robert H. Rubin (HST), Michael J. Cima (MSE), Laurence R. Young (AA), Thomas F. Weiss (EECS), Derek Rowell (ME), Dava J. Newman (AA), Paul L. Penfield, Jr. (EECS), Richard A. Young (Biology), Harold F. Hemond (CEE), Martin A. Schmidt (EECS).
Left to right, kneeling or sitting: Sidney Yip (NE), Douglas A. Lauffenburger (ChE), Roger D. Kamm (ME), Robert E. Cohen (ChE), Jonathan Allen (EECS), Harvey F. Lodish (Biology), Thomas W. Eagar (MSE), Edwin L. Thomas (MSE).
Missing from photograph: David Baltimore (Biology), David M. Chao (Biology), Peter S. Kim (Biology), David C. Page (Biology), Hidde Ploegh (Biology), Herbert H. Sawin (ChE).

Staff

Instructors in charge: Harvey F. Lodish and Richard A. Young.
Teaching Assistant: David M. Chao.

Text

Harvey Lodish, David Baltimore, Arnold Berk, S. Lawrence Zipursky, Paul Matsudaira, and James Darnell, "Molecular Cell Biology," Third Edition, W. H. Freeman and Company, New York; 1995.

Schedule

Monday, June 17
9:00 - 10:30
Rick Young
Lecture: Molecular Biology, DNA, RNA, and protein synthesis, the genetic code

10:30- 12:00
Rick Young
Discussion

12:00- 1:30
Lunch

1:30- 3:00
Harvey Lodish
Lecture: Cell structure, membranes, ATP and glycolysis

3:00- 4:00
Harvey Lodish
Discussion

4:00- 5:00
Gel separation of DNA and proteins
Dave Chao
Location: Project lab 68-077
Agarose gel electrophoresis
SDS-polyacrylamide gel electrophoresis of proteins

Tuesday, June 18
9:00 - 10:30
Rick Young
Lecture: gene regulation

10:30- 12:00
Rick Young
Discussion

12:00- 1:30
Lunch

1:30- 3:00
Peter Kim
Lecture: protein structure

3:00- 4:00
Peter Kim
Discussion

4:00- 5:00
Computer demonstration: looking at and manipulating protein structure
Dave Chao
Location: Whitehead Auditorium
WWW biology resources
3D protein demonstration in auditorium

Wednesday, June 19
9:00 - 10:30
David Page
Lecture: Human genetics and the human genome project

10:30- 12:00
David Page
Discussion

12:00- 1:30
Lunch

1:30- 3:00
Hidde Ploegh
Lecture: Immunology

3:00- 4:00
Hidde Ploegh
Discussion

4:00- 5:00
Lab demonstrations: Microscopy / Tour of Structural Biology Facility / Protein Purification
Escorts: Maureen Murray, Tony Lee, Ellen Gadbois
Each session will be repeated three times for 3 groups of 10:
4:05-4:20
4:25-4:40
4:45-5:00

Microscopy
Yah-Huei Tu, Alex Knight, Jonathan Bogan
Location: Whitehead Institute, WI 447
Optical and electron Microscopy
3D microscopy
Optical tweezers

Tour of the Structural Biology Facility
Jim Berger
Location: Whitehead Institute, WI 627
15 minute tour

Protein Purification
David Chao
Location: Whitehead Institute, WI 543
Protein purification with microscale High Performance Liquid Chromatography

Thursday, June 20
9:00 - 10:30
Harvey Lodish
Lecture: Protein secretion; proteins as drugs

10:30- 12:00
Harvey Lodish
Discussion

12:00- 1:30
Lunch

1:30- 3:00
David Baltimore
Lecture: Control of cell growth/ Cancer

3:00- 4:00
David Baltimore
Discussion

4:00- 5:00
Lab demonstration: growing bacterial and animal cells
Each session will be repeated two times for 2 groups of 15:
4:05-4:25
4:30-5:00

Location 1: Project lab 68-077
Dave Chao
Growth of bacteria and yeast on petri dishes
Growth of mammalian cells

Location 2: Lab 20A-210, Lab 20B-202
Tour of the MIT Biotechnology Processing Engineering Center
Sherry Gu, Robert Balcarcel, Danny Wang

6:30- 10:00
Banquet (All students and lecturers are invited)

Friday, June 21
9:00 - 10:30
Monty Krieger
Lecture: Molecular aspects of human physiology - the digestive and endocrine systems

10:30- 12:00
Monty Krieger
Discussion

12:00- 1:30
Lunch

1:30- 3:00
Monty Krieger
Lecture: Molecular aspects of human physiology - the cardiovascular system and atherosclerosis

3:00- 4:00
Monty Krieger
Discussion

4:00- 5:00
Tour of the Human Genome Center
Bob Steen and Joyce Miller
Location: Genome Center, 1 Kendall Square