{"id":1525,"date":"2013-07-08T19:22:08","date_gmt":"2013-07-08T19:22:08","guid":{"rendered":"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/files\/2013\/07\/shaw_sync_02.png"},"modified":"2013-07-08T19:22:08","modified_gmt":"2013-07-08T19:22:08","slug":"shaw_sync_02","status":"inherit","type":"attachment","link":"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/a-microfluidic-baby-machine-for-cell-synchronization\/shaw_sync_02\/","title":{"rendered":"Figure 2"},"author":370,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"description":{"rendered":"<p class=\"attachment\"><a href='https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-content\/blogs.dir\/22\/files\/2013\/07\/shaw_sync_02.png' rel=\"lightbox[1525]\"><img width=\"300\" height=\"194\" src=\"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-content\/blogs.dir\/22\/files\/2013\/07\/shaw_sync_02-300x194.png\" class=\"attachment-medium size-medium\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-content\/blogs.dir\/22\/files\/2013\/07\/shaw_sync_02-300x194.png 300w, https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-content\/blogs.dir\/22\/files\/2013\/07\/shaw_sync_02-220x142.png 220w, https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-content\/blogs.dir\/22\/files\/2013\/07\/shaw_sync_02.png 676w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n"},"caption":{"rendered":"<p>Figure 2: Operation of the device is controlled entirely by pressure differences. A positive pressure applied at the inlet creates both a pressure differential in the vertical direction across the holes to capture cells and a pressure drop in the horizontal direction to generate lateral flow for the collection of daughter cells. An asynchronous population of cells is fed into the device and captured into the holes; with a constant slow perfusion of cell culture medium, the cells grow within the device. As they divide, the newborn cells, which are not anchored to trapping holes, are carried away in the fluid flow and eluted into a collection chamber. The eluted cells then compose a synchronous population of cells that has just completed cytokinesis and entered the G1 phase of the cell division cycle.<\/p>\n"},"alt_text":"","media_type":"image","mime_type":"image\/png","media_details":{"width":676,"height":438,"file":"2013\/07\/shaw_sync_02.png","sizes":{"thumbnail":{"file":"shaw_sync_02-150x150.png","width":150,"height":150,"mime_type":"image\/png","source_url":"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-content\/blogs.dir\/22\/files\/2013\/07\/shaw_sync_02-150x150.png"},"medium":{"file":"shaw_sync_02-300x194.png","width":300,"height":194,"mime_type":"image\/png","source_url":"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-content\/blogs.dir\/22\/files\/2013\/07\/shaw_sync_02-300x194.png"},"post-thumbnail":{"file":"shaw_sync_02-150x150.png","width":150,"height":150,"mime_type":"image\/png","source_url":"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-content\/blogs.dir\/22\/files\/2013\/07\/shaw_sync_02-150x150.png"},"abstract-thumb":{"file":"shaw_sync_02-220x142.png","width":220,"height":142,"mime_type":"image\/png","source_url":"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-content\/blogs.dir\/22\/files\/2013\/07\/shaw_sync_02-220x142.png"},"full":{"file":"shaw_sync_02.png","width":676,"height":438,"mime_type":"image\/png","source_url":"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-content\/blogs.dir\/22\/files\/2013\/07\/shaw_sync_02.png"}},"image_meta":{"aperture":0,"credit":"","camera":"","caption":"","created_timestamp":0,"copyright":"","focal_length":0,"iso":0,"shutter_speed":0,"title":""}},"post":1523,"source_url":"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-content\/blogs.dir\/22\/files\/2013\/07\/shaw_sync_02.png","_links":{"self":[{"href":"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-json\/wp\/v2\/media\/1525"}],"collection":[{"href":"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-json\/wp\/v2\/media"}],"about":[{"href":"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-json\/wp\/v2\/types\/attachment"}],"author":[{"embeddable":true,"href":"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-json\/wp\/v2\/users\/370"}],"replies":[{"embeddable":true,"href":"https:\/\/mtlsites.mit.edu\/annual_reports\/2013\/wp-json\/wp\/v2\/comments?post=1525"}]}}