{"id":3680,"date":"2011-07-11T14:28:34","date_gmt":"2011-07-11T14:28:34","guid":{"rendered":"https:\/\/mtlsites.mit.edu\/annual_reports\/2011\/?p=3680"},"modified":"2011-07-19T20:54:36","modified_gmt":"2011-07-19T20:54:36","slug":"cell-based-sensors-for-measuring-impact-of-microsystems-on-cell-physiology-2","status":"publish","type":"post","link":"https:\/\/mtlsites.mit.edu\/annual_reports\/2011\/cell-based-sensors-for-measuring-impact-of-microsystems-on-cell-physiology-2\/","title":{"rendered":"Cell-based Sensors for Measuring Impact of Microsystems on Cell Physiology"},"content":{"rendered":"

The use of microsystems to manipulate and study cells in microenvironments is continually increasing.\u00a0 However, along with such increase in usage is also a growing concern regarding the impact of these microsystems on cell physiology.\u00a0 In this project, we are developing a set of cell-based fluorescent sensors to measure the impact of common stresses experienced in microsystems on cell physiology.\u00a0 We are including stress agents commonly found in microsystems (e.g., UV exposure, heat shock, fluid flow, etc.).\u00a0 Each sensor is designed to respond to one particular stress agent but can also be combined for multiplexed analysis of multiple stresses at once, as might be experienced in a typical microsystem. Designed to ease multiplexed analysis, each sensor will use different colors to both indicate the type of sensor and the strength of the signal.<\/p>\n

One sensor in the system will be a heat shock sensor that responds to activation of the heat shock pathway, which is a generalized stress pathway in cells.\u00a0 We are adapting a version of this sensor that we previously reported [1<\/a>] <\/sup> [2<\/a>] <\/sup>, which coupled fluorescent protein expression to activation of heat shock factor 1, from green fluorescent protein (EGFP) to a red fluorescent protein (RFP) and from red (DsRed) to yellow (YPet) for the constitutive color.\u00a0 Figure 1 shows the heat shock sensor response to 15 min heating at 42 \u00baC.\u00a0 Alongside this effort, we are using a multi-flow microfluidic device that can simultaneously apply different flows to cells across a 1000\u00d7 range to understand the behavior of cells in flow [3<\/a>] <\/sup>.\u00a0 Figure 2 is an image of the multi-flow device used to test NIH3T3 mouse fibroblast cells.\u00a0 Cells are seeded in 6 chambers concurrently and exposed to flow for 24 hrs, after which we can extract PCR from each chamber to study the cell response.<\/p>\n\n\t\t