{"id":1599,"date":"2010-07-12T12:05:04","date_gmt":"2010-07-12T16:05:04","guid":{"rendered":"https:\/\/wpmu2.mit.local\/?p=1599"},"modified":"2010-07-21T16:22:23","modified_gmt":"2010-07-21T20:22:23","slug":"scale-down-cell-culture-for-biopharmacueticals","status":"publish","type":"post","link":"https:\/\/wpmu2.mit.local\/scale-down-cell-culture-for-biopharmacueticals\/","title":{"rendered":"Scale-down Cell Culture for Biopharmacueticals"},"content":{"rendered":"

Developing highly productive cell-culture processes is an essential step in the production of biopharmaceutical products.\u00a0 Process development involves experimentally determining the combination of cell line, culture medium composition, and process parameters that will produce the highest quality and quantity of product.\u00a0 Scale-down models for large-scale bioreactors are essential for cell-culture process development in order to achieve the throughput necessary to conduct a sufficient number of experiments for a reasonable cost.\u00a0 Bioreactor systems based on 24 well plates [1<\/a>]<\/sup> and a highly automated robotic system with passive microreactors [2<\/a>]<\/sup> have recently been developed in order to overcome the shortcomings of traditional shake-flask and bench-scale stirred-tank-based scale-down models.\u00a0 However, in these systems mixing and fluid-handling require external machinery\/robotics or manual intervention.\u00a0 We are using an alternative approach that integrates fluid-handling and mixing capabilities into the bioreactor device utilizing previously developed fluid injectors and mixing devices [3<\/a>]<\/sup>.\u00a0 Figure 1 shows a schematic view and photographs of the mammalian-cell-culture reactor.\u00a0 It is fabricated with injection-molded and machined biocompatible polycarbonate layers and an actuated silicone membrane.\u00a0 Initial CHO cell cultures show comparable growth and viability to shake flask cultures.<\/p>\n\n\t\t