{"id":1479,"date":"2010-07-08T11:36:52","date_gmt":"2010-07-08T15:36:52","guid":{"rendered":"https:\/\/wpmu2.mit.local\/?p=1479"},"modified":"2010-07-12T15:15:41","modified_gmt":"2010-07-12T19:15:41","slug":"size-selective-sorting-of-cells-using-templated-assembly-by-selective-removal","status":"publish","type":"post","link":"https:\/\/wpmu2.mit.local\/size-selective-sorting-of-cells-using-templated-assembly-by-selective-removal\/","title":{"rendered":"Size-Selective Sorting of Cells using Templated Assembly by Selective Removal"},"content":{"rendered":"
\"Figure<\/a>

Figure 1: Schematic illustration of the experimental set-up for TASR including the assembly fluid (in which variable chemistry and shape-matching are implemented through the choice of materials and component\/template geometry) and the 1.7-MHz ultrasonic transducer that introduces mechanical forces to the selective removal system.<\/p><\/div>\n

This work presents the size-selective sorting of single biological cells using Templated Assembly by Selective Removal (TASR). We have demonstrated the selective self-assembly of single SF9 cells (clonal isolate derived from Spodoptera frugiperda<\/em> IPLB-Sf21-AE cells) into patterned hemispherical sites on rigid assembly templates using TASR. Experimental success with SF9 cells, which are nearly spherical and resistant to shear, suggests that self-assembly using TASR can also be extended to other cells and biological materials that are spherical.\u00a0 Examples include white blood cells and, in general, cells that maintain a well-defined morphology for short durations when dispersed in culture media, agitated mildly using megasonic excitation, and allowed to settle on a patterned substrate. <\/strong>Therefore, <\/strong>TASR-based biological self-assembly holds potential for several applications, such as cell-sorting for medical research or diagnostics, or isolation of single cells for studying their biological and mechanical behavior. <\/strong><\/p>\n

\"Figure<\/a>

Figure 2: Optical micrograph of SF9 insect cells with mean diameter of 15 microns, stained with methylene blue and self-assembled (a) selectively into patterned hemispherical sites with alternating diameters of 12 and 22 microns (b) uniformly into patterned hemispherical sites with a diameter of 15 microns on a templated silicon surface using TASR.<\/p><\/div>\n

In TASR, the system\u2019s free energy is minimized when objects assemble in holes that match their shapes and sizes on the template\u2019s surface (Figure 1). A combination of chemical and mechanical effects selectively removes objects from poorly matched holes. Previous work on TASR has shown that microcomponents made from relatively rigid materials such as silica [1<\/a>]<\/sup> [2<\/a>]<\/sup> and deformable materials like polystyrene [3<\/a>]<\/sup> can be assembled effectively on similarly rigid patterned templates using this technique. In an extension of the application of TASR to biological systems, SF9 cells (which come in a range of sizes with a mean diameter of 15 microns) were successfully assembled using TASR onto patterned silicon templates. The assembly sites comprised holes with nearly hemispherical profiles etched in a silicon substrate using DRIE. Figure 2 shows optical micrographs of the assembly and demonstrates the size-selectivity of the process as well as the high yield of cell assembly using this technique.<\/p>\n

\r\nReferences
  1. S. Jung and C. Livermore, \u201cAchieving selective assembly with template topography and ultrasonically induced fluid forces,\u201d Nanoletters, <\/em>vol. 5, no. 11, pp. 2188-94, 2005. [↩<\/a>]<\/li>
  2. F. Eid, S. Jung, and C. Livermore, \u201cTemplated assembly by selective removal : simultaneous, selective assembly and model verification,\u201d Nanotechnology<\/em> vol. 19 p. 285602, 2008. [↩<\/a>]<\/li>
  3. G. Agarwal, A. Servi, F. Eid, and C. Livermore, \u201cShape Selective Assembly in Deformable Systems using Templated Assembly by Selective Removal,\u201d Proc. of the Foundations of Nanoscience Conference- Self-Assembled Architectures and Devices<\/em>, Snowbird, Utah, 2009. [↩<\/a>]<\/li><\/ol><\/div>","protected":false},"excerpt":{"rendered":"

    This work presents the size-selective sorting of single biological cells using Templated Assembly by Selective Removal (TASR). We have demonstrated…<\/p>\n<\/div>","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[29],"tags":[57,4155],"_links":{"self":[{"href":"https:\/\/wpmu2.mit.local\/wp-json\/wp\/v2\/posts\/1479"}],"collection":[{"href":"https:\/\/wpmu2.mit.local\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wpmu2.mit.local\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wpmu2.mit.local\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wpmu2.mit.local\/wp-json\/wp\/v2\/comments?post=1479"}],"version-history":[{"count":6,"href":"https:\/\/wpmu2.mit.local\/wp-json\/wp\/v2\/posts\/1479\/revisions"}],"predecessor-version":[{"id":2243,"href":"https:\/\/wpmu2.mit.local\/wp-json\/wp\/v2\/posts\/1479\/revisions\/2243"}],"wp:attachment":[{"href":"https:\/\/wpmu2.mit.local\/wp-json\/wp\/v2\/media?parent=1479"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wpmu2.mit.local\/wp-json\/wp\/v2\/categories?post=1479"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wpmu2.mit.local\/wp-json\/wp\/v2\/tags?post=1479"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}